Culture and Management of Scylla Species (CAMS)

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Work Package 1: Bacterial Disease Control

Objective: To improve the reliability and sustainability of crab hatchery systems through the use of probiotic bacteria as an alternative to microbials in disease control
 

VIETNAM 

Manipulation of microbial populations

Failure to control pathogens in the hatchery using the conventional methods of formalin and chlorination has to the development of alternative methods of improving the bacterial populations in the rearing water. A new integrated rotifer-tilapia-Chlorella system is now being used for the production of rotifers fed on Chlorella produced in tilapia tanks. Initial results suggest that this green water system seems to have a good bacterial community with low Vibrio count and resulted in highest zoeal survival rates when compared with clear water larval rearing systems.

Integrated tilapia-Chlorella-rotifer system (5m3 of tilapia-Chlorella & 0.5m3 of rotifers)


Probiotic bacteria

Forty-one possible strains of probiotic bacteria have been isolated from the culture water in the crab larviculture system. Bacteria were selected for anti-microbial activity and for dominance in the culture medium. Selected strains are maintained in deep freezer for later screening.


References

Keenan, C. P., Davie, P. J. F. and Mann, D. L. (1998). A revision of the genus Scylla de Haan, 1833 (Crustacea : Decapoda : Brachyura : Portunidae). Raffles Bulletin of Zoology 46, 217-245.

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